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Statistical analysis For animal studies, sample sizes ( n = 5 per group, 3 in the tirzepatide-treated group) were determined based on prior in-house experience and previous studies using similar mouse models to ensure adequate power to detect biologically relevant effects
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This can help when your dose needs to be changed
The numbers of precursors/proteins were obtained based on filtering the library for precursors within the charge range from 2 to 4 and the mass range adjusted to our acquisition scheme
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