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In addition, 1,25(OH)2D3 induces the release of neurotrophin and synthesis of Ca 2+ -binding proteins such as parvalbumin, inhibits the synthesis of inducible iNOS, macrophage colony-stimulating factor, TNF-, down-regulates L-type voltage sensitive Ca 2+ channel (LVSCC), and upregulates -glutamyl transpeptidase activity, which are also reported to be the potential neuroprotective mechanisms of VD in PD (Figure 2A) (9193)

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boulardii achieved an escape frequency below 10 9 and remained stable for over 100 generations, underscoring the feasibility of such systems for future live biotherapeutic applications (Maneira et al., 2025)