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The sections were blocked with 5% goat serum (Jackson ImmunoResearch, 005-000-121) in antibody diluent (Dako) for 30 min and incubated overnight at 4 C with following antibodies: insulin, glucagon, -H2AX, MOTS-c or IL-1 (1:100 of antibody concentration)

Structurally it is the GLP-1 backbone with substitutions that resist enzymatic breakdown plus a fatty-acid chain that binds albumin, slowing clearance

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[DOI] [PMC free article] [PubMed] [Google Scholar] 11.Wilding J.P.H., Batterham R.L., Calanna S., Davies M., Van Gaal L.F., Lingvay I., McGowan B.M., Rosenstock J., Tran M.T.D., Wadden T.A., et al