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In human cell culture, Jakubowski (1997) showed that the thiolactone produced by an altered homocysteine metabolism may be incorporated into cellular and extracellular protein through lysine acylation by its activated carboxyl group
These are inexpensive and available from laboratory supply companies
Plasma which was not deproteinized prior to storage was removed from storage (80 o C and 4 o C, respectively) and mixed with an equal volume of cold 5% SSA and incubated for 10 min at 4 o C (on ice)
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