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The PVDF membrane was subsequently washed (5 min 5) with tris-buffered saline with tween (TBST) buffer and then incubated with the appropriate secondary antibody (Peroxidase-conjugated goat anti-rabbit IgG (H + L), at a dilution of 1:5000) in a 5% bovine serum albumin solution for 1 h at 25 C

Modulation of Growth Hormone Fragment Pathways : AOD-9604 represents the modified fragment 177-191 of human growth hormone , designed to retain the fat-burning properties of HGH without its systemic growth or insulin-like effects

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Glutathione's antioxidant properties also play a role in reducing oxidative stress and DNA damage caused by alcohol