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For Oil Red O Staining, liver tissues were frozen in Tissue-Tek CRYO-OCT (Fisher Scientific) and cryostat tissue sections were cut at 7 m thicknesses, fixed with 10% formalin for 10 min, and stained following an Oil Red O procedure

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Out of all urate associated loci, only three (INHBB, HNF4G and UBE2Q2) are not formally associated with gout