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Gad1 rats: -6.30 mm A/P, +or- 0.50 mm M/L, and -8.49 mm D/V) and secured to the skull with Metabond cement (Parkell) and dental acrylic

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They have emerged as key therapeutic targets in diabetes and obesity research , inspiring the development of dual and triple receptor agonists that synergistically enhance glycemic control, promote weight loss, and improve overall metabolic health

Mice were given bilateral injections into the substantia nigra with AAV expressing Cre - EGFP under the control of a human synapsin-1 promoter (AAV- hSyn - Cre - EGFP ), as described above, to obtain a strain of A53T with conditional knockdown of Gpx4 (CKD)