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Elution steps were performed in an according buffer (50 mM Tris-HCl, 140 mM NaCl, 2 mM DDT, 5-10 mM reduced glutathione, pH 8.0)

PMID: 31832627

However, it seems that long-term L-carnitine intake eventually converts to a metabolite called trimethylamine N-oxide (TMAO) (9799), and increased circulating levels of this metabolite can increase the prevalence of diabetes (54% per 5 mol/L increment of plasma TMAO) (100), nonetheless one study showed that increased TMAO plasma level by 30 mol/L after 24 weeks of L-carnitine supplementation was not related to glucose, insulin and HOMA-IR levels changes (97)

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