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Next, we used two popular immunotherapies, anti-PD-1 mAb (PM) therapy and WCV therapy, and investigated whether they worsened or rescued the ovarian damage caused by cancer

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Stained images were observed and captured using an inverted microscope ( Short-chain fatty acid (SCFA) analysis Approximately 0.1 g of intestinal content from each mouse was accurately weighed and homogenized with 1 mL of phosphate-buffered saline (PBS) to prepare a 10% (w/v) fecal suspension