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Setting up a laboratory in a prefab on the roof of some old buildings at the back of the Royal Free Hospital in 1960, Samols persuaded Vincent Marks (figure 12), a junior doctor with an interest in carbohydrate metabolism, to help him demonstrate the clinical use of RIA

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After incubation with TruStain fcX -mouse CD16/32 blocking antibody (BioLegend), staining of different surface markers (see Table 1) was performed for 20 min at 4 C followed by a washing step with PBS/1% FCS