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Generation of closed SpyLocks SpyLock constructs (including BiLockCatcher fusions) were incubated in PBS/2.5 mM TCEP for one hour, followed by buffer-exchange (MiniTrap G-25 columns, Cytiva) into DTNB-labeling buffer (100 mM sodium phosphate, 1 mM EDTA, pH 8.0) or biotin-labeling buffer (PBS pH 7.4, 1 mM EDTA)

The daily administration allows for rapid dose modifications if side effects occur

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2 Overview of osteoarthritis and ferroptosis 2.1 Pathogenic mechanisms of osteoarthritis OA is a common degenerative joint disease marked by gradual cartilage degradation, destruction of subchondral bone structure, synovial inflammation, and clinical symptoms such as joint pain