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The VEGFR2-Akt-eNOS signaling pathway might be activated without the need for other known ligands or shear stress, controlling vasomotor tone and the activation of the Src-Caveolin-1-eNOS pathway [60,61]

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In DMSO-treated control, only few endothelial cells migrated from the upper to the lower chamber through the membrane after 12-hour incubation, when the lower chamber contained culture medium supplemented with 10% FBS as a chemoattractant