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The metabolism of peptides and proteins usually starts from the action of endopeptidase and then undergoes multi-step enzymatic degradation to produce the final metabolite amino acids, which enter the amino acid pool in vivo ( 3 H]BPC157, and their structures were predicted by LC-MS/MS molecular weight identification and comparison with standards

10.1097/AOG.0000000000002469 45 FanC.LiX.XiaoZ.ZhaoY.LiangH.WangB.et al (2017)

Phase separation was induced by adding 300 L of ice-cold water, followed by another 3 minutes of sonication, 15 minutes of vortexing at 15C and 1000 rpm, and then centrifugation at 21,000 g and 4C for 15 minutes

Mann DA (2014) Epigenetics in liver disease