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All lenses were incubated for 3 to 4 days in 2 mL of M199 medium (Sigma-Aldrich) containing 0.1% BSA and 30 mM Galactose using an incubator set at 5% CO 2 and 37 C to induce opacity, as previously reported 30

Furthermore, changes in the number of CTC after treatment were more strongly associated with survival than were changes in PSA at 4, 8 and 12 weeks post treatment (de Bono et al, 2008

This classification leaves patients to shoulder the full cost, which can be prohibitively expensive

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