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Previously in our lab, we analyzed the interaction of pseudorabies virus (PRV, diameter = 250 nm) with porcine respiratory mucus using SPT [194]

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Single cell suspensions of LN cells were prepared by gentle mechanical disaggregation through 200 micron-mesh stainless steel gauze and resuspended in RPMI-1640 (Life technologies corporation, Grand Island, NY, USA) supplemented with fetal calf serum (Life technologies corporation, Grand Island, NY, USA) (10%), HEPES (Sigma-Aldrich, St

Dehydration overnight can reduce blood volume by 1-2%, causing blood vessels to constrict and triggering tension-type headachesthe body loses approximately 1-2 pounds of water through breathing and sweating during 8 hours of sleep