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However, when hyperhomocysteinemia was simulated and a cysteine-block applied (Figure 6B), it prevented a significant elevation in cytosolic GSH levels: GSH levels deviate by only about 10% from the steady-state at nominal values of and

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a , Scheme of the in vitro sgRNA competition assay performed in HEK-293T SLC25A39 knockout cells expressing a vector control or SLC25A39 cDNA transduced with a pool of 5 control sgRNAs (sgControl, gray) and sgRNAs targeting TXNRD2 (pink) and SLC25A40 (green) (left)