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Primary antibodies used included goat anti-choline acetyltransferase (ChAT, 1:1000 dilution, AB144P, Millipore) and rabbit anti-c-Fos (1:1000 dilution, 2250 S, Cell Signaling Technology)

Earlier initiation of SGLT2is or GLP-1RAs may provide cardiometabolic protection before the development of advanced disease, whereas longer-standing disease often necessitates combination therapy due to progressive -cell dysfunction and accumulated risk [8]

When an infection occurs due to bacteria or a virus, lymph nodes can become swollen, often signalling the onset of illness, such as a cold