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mitochondrial dysfunction leads to cortical under-conneectivity and cognitive impairment

Cells were incubated with the agonist for 15 min (except in the case of KRAS assays, where the incubation time was 60 min), and bioluminescence resonance energy transfer (BRET) measurements were taken using a Victor X4 plate reader (PerkinElmer) immediately after the addition of coelenterazine h (final concentration: 1 M)

Indirect Evidence for A Role of Redox Dysregulation in Als As well as the studies described above, there is also evidence that cellular processes associated with redox homeostasis are dysregulated in ALS (Figure 2)