Cells were seeded on collagen-coated plates (250 g/ml rat collagen, Roche) at a density of 150,000 cells/cm 2 and cultivated for 3 h in Williams E medium (PAN Biotech) containing penicillin (100 U/ml, Gibco), streptomycin (0.1 mg/ml, Gibco), gentamycin (10 M, PAN Biotech), dexamethasone (100 nM, Sigma), stable l-glutamine (2 mM, Sigma), insulin supplement (2 ng/ml, Sigma), and 10% Sera Plus (PAN Biotech)
The recommended dietary allowance (RDA) range is as follows [3]: Ages 1418, both male and female 2.4 mcg Ages 19+, both male and female 2.4 mcg Pregnant individuals Between 2.62.8 mcg Again, individual needs may differ, and vitamin B12 requirements in pregnancy are very important, so its best to speak with a healthcare professional about the exact amount of B12 you need for optimal health
PMID:7342494
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