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Despite this, the plasma mGSTP1 assay has several advantages: (1) the blood for a mGSTP1 assay can be collected in a standard EDTA tube rather than a specialised CTC tube (CellSave Preservative Tube, Johnson & Johnson), (2) the sample for a mGSTP1 assay can be spun and frozen for later assaying, as DNA methylation is a stable marker, as opposed to the CTC assay that must be run within 96 h of blood collection and (3) the mGSTP1 assay uses standard RT-PCR assay equipment available in most diagnostic laboratories, rather than requiring a specialised machine

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