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PloS One 10

doi: 10.1073/pnas.0909927107 54 MagnaMPisetskyDS

Quantitative real-time PCR Total RNA was isolated from tissue or cells using Qiazol and RNeasy mini kit (Qiagen, Venlo, Netherlands) according to manufacturers instructions and with 15 min on-column DNase digestion (Qiagen, Venlo, Netherlands)

A study in adults aged 70 and older showed increased heteroplasmy linked to an increased depression score.[ref] Rare mitochondrial mutations that cause mitochondrial diseases are also linked to major depressive disorder.[ref] In fact, one study of people with hereditary (genetic) mitochondrial diseases showed that 54% had major depressive disorder and 17% had bipolar disorder.[ref] Outside of the mitochondrial genome, genes in the nuclear genome that code for proteins in the mitochondria are also linked to depression