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Zinc-mediated inhibition of cyclic nucleotide phosphodiesterase activity and expression suppresses TNF-alpha and IL-1 beta production in monocytes by elevation of guanosine 3,5-cyclic monophosphate

The mice were housed in conditions were controlled with a temperature range of 2024 C, humidity maintained at 5060%, and a 12-h lightdark cycle, with free access to food and water

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The retinal damage procedure included L-NAME administration in a total dose of 5 mg/kg, which was given by retrobulbar application in each eye, with 0.5 mg/0.1 ml saline per eye