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Paradoxical Decreases in Heart Rate at Low Sub-Therapeutic Doses of Atropine (1) a central vagotonic mechanism , whereby low-dose atropine blocks inhibitory M1 muscarinic receptors in the central nervous system or parasympathetic ganglia, disinhibiting vagal outflow and increasing parasympathetic tone to the sinoatrial node (Kotona et al, 1977

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Cell-bound IFN-, CD11b, CD8 and CD4 expression in LLC single tumor cells upon the different treatments was analyzed by flow cytometry using the antibodies: PE/Cy7 anti-mouse IFN-y (Biolegend, 505825), PE-Cy7 Rat Anti-Mouse CD11b (BD Pharmingen, 552850), V500 Rat anti-mouse CD8a, clone 536.7 (BD Horizon, 560776), FITC conjugated anti-mouse CD4, clone RM45 (Ebioscience, 11-0042-85) according to manufacturers instructions

The ability of the nanoparticles to store and transport oxygen was studied using a dissolved oxygen analyzer