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An animal study revealed that when DSIP was directly injected into the bulbo-mesencephalo-thalamic recruitment system, it induced slow-wave sleep and Naloxone-reversed sleep spindle activity

To the other portion, add 2.0 mL of a standard potassium chloride solution (prepared by dissolving 0.191 g of potassium chloride, previously dried at 105C for 2 hours, in water in a 1000 mL volumetric flask, diluting to volume with water, and mixing well

however, in cases of severe damage, such as complex DNA breaks, these foci become persistent [18]