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As the HGF-Met receptor pathway is essential for cutaneous wound healing and re-epithelialization 30 , we next performed a wound-healing assay, using NHEK cells (Fig

Considering again that the myogenic stem cell activator HGF is found in an ECM-bound state 19,20,21,22,26 and undergoes nitration of tyrosine residues (Y198 and Y250) with aging 32,32,62 and muscle disuse (Kuwakdo et al., 2025), the present in vivo experiments provide a robust platform showing the potential for LASSS that could efficiently reduce or delay the nitration-dysfunction of ECM-bound HGF under peroxynitrite-generating conditions

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This delivery method offers several key benefits