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Samples were loaded into standard Monolith capillaries and measured using the Monolith 2020 pico-RED instrument (NanoTemper) at 100% excitation and medium microscale thermophoresis power at 25 C

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It mediates most of the systemic effects of growth hormone through agonism at the IGF-1 receptor (IGF-1R), a transmembrane tyrosine-kinase receptor expressed on most tissues