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Estimation of liver function parameters Aspartate aminotransferase (AST), alanine aminotransferase (ALT) were assayed in serum samples obtained from all groups of mice by a colorimetric method of Reitman and Frankel (Reitman and Frankel, 1957), while albumin was assessed according to the method of Pinnell and Northam (Pinnell and Northam, 1978) using commercially available kits (Diamond Diagnostic, Egypt) and the Erba CHEM-7 device (ERBA Diagnostics, India)

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Serum estradiol after single dose hCG administration correlates with Leydig cell reserve in hypogonadal men : reassessment of the hCG stimulation test

This situation is incompatible with life unless there is mixing between the two circulations