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These facts led to the search for and design of GST inhibitors, including their synthetic analogues and glutathione conjugates, however, most of the existing inhibitors are either too toxic to be used in vivo or are effective only in vitro [14, 15]

The quantification of DNMT1, DNMT3a, and DNMT3b expression levels was performed using real-time PCR on an Applied Biosystems 7500 system

Importantly, 70-80% of participants achieved at least 10% weight loss (the threshold for clinically meaningful benefits), and 50-60% achieved at least 20% weight loss (approaching surgical weight loss results without surgery)