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For morphometric analysis of granuloma and immune cell infiltration, the H&E stained mice lung sections were analyzed using Path Scan Enabler-5 (Mayer Scientific, TX, United States) followed by morphometric determination of the area of lung involved in granulomatous response using Sigma Scan Pro (Systat Software Inc., CA, United States) as described previously ( Quantification of Glutathione Levels GSH levels were measured in the lung lysates of untreated, 40 mM L-GSH, RIF, and RIF +40 mM GSH treated diabetic mice at 4-weeks and 8-weeks post-infection

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Metabolic syndrome, characterized by abdominal obesity, elevated triglycerides, low HDL-C, hypertension, and insulin resistance, confers increased cardiovascular risk