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For studies that are solely focused on investigating the effect of any intervention on the APAP hepatotoxicity phase, the inclusion of regeneration phase time points should also be considered to rule out any deleterious effects of that intervention on liver regeneration and overall recovery

Then, all the samples were then transferred to a 10% formalin-neutral buffer solution and embedded in paraffin

Inappropriate iron supplementation or excessive intake of iron supplements often leads to ROS accumulation through the Fenton and Haber-Weiss reactions, triggering oxidative stress, LPO, damage to intestinal epithelial cells, and even cell death, which disrupts the intestinal mucosal barrier function