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In other studies, prophylactic immunization with the hydatid cyst wall protein bands (~ 27/28 kDa), that showed cross-reactivity with breast cancer patients sera, or crude hydatid cyst wall antigens (laminated layer) caused significant inhibition of 4T1 breast tumor growth, decrease of metastasis, and prolongation of the tumor-bearing mice survival time through a rise in the levels of IL-2, TNF-, IFN-, and IL-4 [51, 181]

Furthermore, each batch undergoes testing for endotoxins using the Limulus Amebocyte Lysate (LAL) assay to ensure it is free from bacterial contaminants that could interfere with experimental results, particularly in cell-based or in vivo studies

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