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Intracellular Fe 2+ determination After different pretreatments, a FerroOrange assay kit (F374, Dojindo, China) was used to determine the Fe 2+ levels in GC cells

Comparison to Existing Therapies Placing PT-141 in context requires comparing it with the existing standard therapies for male sexual dysfunction

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Guanosine and uroguanosine, which target guanosine cyclase (GC-C) receptors in the intestine and kidney, lead to elevated intracellular cGMP concentrations, and high GC-C expression in L cells is associated with regulation of GLP-1 release (Friedlander et al., 2011)