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While high-throughput screening methods are valuable, the method developed here takes a different approach and focuses on the chromatographic separation of several key acylcarnitines that are used for diagnosing different disorders [2]
For example, in 2% albumin condition, the IC 50 is 5.39 when lipidation occurs at position 27, yet at position 26 it reaches 357.2 It is possible that lipidation at certain sites can create steric hindrance that does not allow GLP-1Rs to capture ligands directly from the albumin, but only to trap free ligands released by the albumin
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M.PapagiannakopoulosT.MoreiraA
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