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Embodiment 2 [0048] Example 2: Synthesis of Intermediate I [0049] [0050] R 1 =Fmoc, R 3 =Fmoc [0051] Weigh 50.00g Fmoc-L-His(Fmoc)-OH into 500ml dichloromethane, stir, add 21.55g DIPEA (2.00eq) to the reaction flask, stir to dissolve, and add 14.21g DIC (1.35g) to the reaction flask in turn eq), 15.21g HOBt (1.35eq), 19.58g 2-aminoisobutyric acid tert-butyl ester hydrochloride (1.20eq), stirred for 6 hours, the reaction was complete, the reaction solution was concentrated, the residue was dissolved in 300ml EA, and the organic phase was dissolved in 300ml Washed twice with purified water, washed once with 300ml of saturated aqueous sodium chloride solution, separated, collected the organic phase, concentrated the organic phase to 100g, controlled the temperature to 0-10C, added the concentrated dropwise to 750ml of petroleum ether for crystallization, and finished the dropwise addition After that, the mixture was kept at 0-10C and stirred for 30 minutes, filtered, and the fil

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Received date: 02-03-2024 Accepted date: 19-05-2024 Published date: 15-09-2024 Downloads Article Details Issue Section This work is licensed under a Creative Commons Attribution 4.0 International License