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These cells were maintained in high glucose Dulbeccos Modification of Eagles Medium (DMEM, Life Technologies, Carlsbad, CA) supplemented with 10% (v/v) heat inactivated fetal bovine serum, 100 units/ml penicillin/streptomycin, and 2 mM L-glutamine (Life Technologies, Carlsbad, CA) at 37C in a humidified air atmosphere at 5% CO 2

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Alternatively, selective exposure of reactive cysteines, especially those located within protein functional pockets or solvent-accessible regions, may confer differential susceptibility to oxidation (5860)

Scientific evidence suggests that KD may alter the composition of the gut microbiota, reducing pro-inflammatory Proteobacteria and increasing the ratio of Bacteroidetes to Firmicutes , which could potentially be beneficial in inflammatory skin diseases