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A deficiency can happen for a few different reasons, and its not always as simple as just changing what you eat

A deficiency or lack of Vitamin B12 is quite common in the over 65s, those diagnosed with pernicious anaemia, and in anyone who follows a vegan, strict vegetarian, or extreme/fad/restricted diet for a long time without taking a B12 supplement or eating fortified foods

Pregnant or breastfeeding women who suspect B12 deficiency should have their levels tested by their prenatal care provider, who can recommend the appropriate supplementation route and dosage

If you plan to use the entire vial in a single instance, sterile water is acceptable, but for standard research protocols, bac water is essential