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7 Conclusions HTY is a phenolic compound found mainly in olive oil, and it has gained considerable attention as a bioactive natural compound with potential health-related benefits

Their efficacy is assessed through in vitro (e.g., DPPH, FRAP), in situ, ex vivo, and in vivo models, as well as animal and human clinical trials, each offering unique advantages but facing challenges in standardization and physiological relevance

pepsin digests with ABC (100 mM), prior to solid-phase extraction (SPE) on 1 cc (30 mg) OASIS HLB cartridges (Waters, Milford, MA) eluted with 100% MeOH (1 mL)

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