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Clinicians can prescribe GLP-1s off-label for people with BMIs below 27, but insurance rarely picks up the cost

After all tests were completed, spleen and brain were collected for further analysis

This specific localization within the GCL is of profound clinical relevance, as retinal ganglion cells are the primary neurons that degenerate in glaucoma and are also critically affected during the early neurodegenerative stages of diabetic retinopathy [38, 39]

This fragment was digested with EcoRI (NEB) and ligated into pGex-6p1 (GE Healthcare) prepared by digestion with BamHI and blunting with Mung Bean Nuclease (NEB) to generate pJB-GST-HTS