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MDA-MB-231 and MDA-MB-468 cells were cultured in Gibco's Leibovitz's L-15 medium with 10% FBS (Thermo Fisher Scientific, Inc.)

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However, reports published on Scopus, PubMed, and Web of Science were studied which gives us important information that TMAO, the gut metabolite, is not only involved in causing atherosclerosis but its high levels in the blood can be a serious threat to human life due to its association with various other ailments

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