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Before the experiment, the cells were loaded with 0.2% Fluo-4 AM, which was dissolved in HEPES-buffered Hanks Balanced Salt Solution (HBSS, Thermofisher) containing 1 mM CaCl2, 1 mM MgCl2, and 0.5% probenecid (Thermofisher) for one hour

All animal procedures were performed in accordance with the guidelines of the Committee on Animal Care (Kyushu University)

To understand what neuralgia is, it is first important to look at how the nervous system works and what role it plays in the perception of pain