The effectiveness of siRNA knockdown was determined by measuring the expression of CrAT using (A) reverse transcription polymerase chain reaction (RT-PCR), (B) Western blotting, (C) CrAT mRNA level, mitochondrial CrAT protein level, sterol regulatory element-binding protein-1 (SREBP-1), phosphorylated 5AMP-activated protein kinase (pAMPK), peroxisome proliferator-activated receptor alpha (PPAR), phosphorylated acetyl-CoA carboxylase (pACC), uncoupling protein 2 (UCP2), carnitine palmitoyltransferase-1 (CPT-1), phosphoenolpyruvate carboxykinase (PEPCK), and glucose 6-phosphatase (G6Pase) were assessed by Western blotting
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Innovatively, the authors employed PMVL-pretreated tumor cells as a vaccine, which effectively inhibited heterologous 4T1 tumor growth in immunized mice (Fig
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