The fluorescence intensity of SF650T-labeled Halo7-Rac1 released from the EVs in the endosomes into the cytoplasm in the recipient cells was measured as follows: puncta of the Halo7-Rac1, as Halo7-Rac1 remained in the EVs in the endosomes, were detected using Analyze Particles plugin of Image J as the particles with a threshold of more than 3000
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Serious adverse effects (SAEs) were reported in two (3.08%, chest pain and lumbar spinal stenosis) and one patient (1.52%, spinal osteoarthritis) in the Met+Sita and Met+Sita+Acarb group, respectively
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