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A UK EVM found that a small clinical trial in Alzheimers patients revealed no adverse effects of thiamineHCl at daily oral intakes of 6000 to 8000mg for five to six months

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Briefly, the slides were incubated with 3% H 2 O 2 to eliminate endogenous peroxidase activity and then blocked with 5% BSA followed first by incubation with primary antibodies against VEGFA (1:200) and anti-IL8 (1:200) overnight at 4 C, and second by incubation with a secondary antibody