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The homogenate was filtered through a 100-m cell strainer and centrifuged at 500 g , followed by transfer of the floated adipocytes to an Eppendorf tube for RNA isolation

AST modulates neuroinflammation by downregulating NF-B signaling and inhibiting microglial activation, leading to reduced secretion of pro-inflammatory cytokines such as IL-1, IL-6, and TNF-