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RAGE engagement triggers NFkB, leading to generation of reactive oxygen species with mitochondrial dysfunction in both podocytes and endothelial cells, fostering inflammation and fibrosis

For experiments in 384-well plates, 500 cells were seeded per well, and after 24 h, the medium was switched using a series of 4 aspirationwash steps with the desired final medium using Multidrop Combi (Thermo)