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Waddington CH

doi:10.1016/j.cell.2010.06.028

International Journal of Impotence Research .Read source (2004)

On the day of assay 2 g per well of the cell membrane was incubated with furimazine (1:1000 dilution from stock) in assay buffer (1 HBSS, 10 mM HEPES, 0.1% (w/v) BSA, 1 P8340 protease inhibitor cocktail, 1 mM DTT and 0.1 mM PMSF, pH 7.4)