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however, most results were obtained via single-center observational and retrospective studies and lack information on confounding factors, such as duration of PPI exposure, antibiotic intake, and dietary patterns
Primary Tumor Growth Spontaneous Metastases Cell lines derived from 4T1 primary tumors (see above) were stably transduced with pLKO.1 vectors (Control and pooled shRNAs targeting Gstt1 ), harvested by trypsinization, washed twice in PBS, resuspended at 1 x 10 4 in 90ul PBS and 10% Matrigel (Corning) and injected into the 4 th inguinal mammary fat pad of BALB/c mice (Jackson Laboratory) to establish mammary tumors